Anti-TRAF6 antibody

• Supplier: Abcam
• Catalog: ab13853
• Prices: $398.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide: DQSEAPVRQNHEEI , corresponding to amino acids 436-449 of Human TRAF6
• Description: Rabbit Polyclonal
• Gene: TRAF6
• Conjugate: Unconjugated

Product images

IHC image of ab13853 staining in human colonic carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13853, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab13853 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13853, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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The UL144 gene product of human cytomegalovirus activates NFkappaB via a - The UL144 gene product of human cytomegalovirus activates NFkappaB via a

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CCR4 is a key modulator of innate immune responses. - CCR4 is a key modulator of innate immune responses.

Ness TL, Ewing JL, Hogaboam CM, Kunkel SL. J Immunol. 2006 Dec 1;177(11):7531-9.