Anti-Transferrin Receptor antibody

• Supplier: Abcam
• Catalog: ab84036
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-F IHC-P
• Species Reactivities: Mouse, Human, Dog, Pig, Orangutan
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Transferrin Receptor
• Description: Rabbit Polyclonal
• Gene: TFRC
• Conjugate: Unconjugated

Product images

All lanes : Anti-Transferrin Receptor antibody (ab84036) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab84036 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84036, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.

ab84036 immunohistochemical staining in the rat brain. Sampled were fixed with formaldehyade and heat-mediated antigen retrieval was performed with citric acid. Tissue sections were blocked in 1% BSA for 10 minutes at 21°C before incubation with ab84036 (1/6000) for 16 hours at 21°C. A biotin conjugated goat anti-rabbit IgG secondary was used at 1/250.See Abreview

Anti-Transferrin Receptor antibody (ab84036) at 1 µg/ml + Spleen (Mouse) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab84036 staining Transferrin Receptor in canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% saponin then incubated with ab84036 at a 1/100 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.See Abreview

ab84036 staining mouse liver sections by IHC-FrFl. The animal was perfused with 4% paraformaldehyde, further post fixed with 4% paraformaldehyde overnight and prepared for free floating sectioning. Staining with ab84036 at a 1/200 dilution in 0.1% TritonX with 0.1x PBS and 10% donkey was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody was used as the secondary antibody.See Abreview

Product References

Transferrin receptor specific nanocarriers conjugated with functional 7peptide - Transferrin receptor specific nanocarriers conjugated with functional 7peptide

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Zhang X, Cruz FD, Terry M, Remotti F, Matushansky I. Oncogene. 2013 May 2;32(18):2249-60, 2260.e1-21.