Anti-Transportin 1 antibody [D45]
| Name | Anti-Transportin 1 antibody [D45] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10303 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | D45 |
| Applications | FC ICC/IF ICC/IF IHC-P ELISA WB IP |
| Species Reactivities | Mouse, Rabbit, Human, Monkey |
| Antigen | Recombinant his-Transportin 1 (Human) |
| Description | Mouse Monoclonal |
| Gene | TNPO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Transportin 1 antibody [D45] (ab10303) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/14099_Transportin-1-Primary-antibodies-ab10303-4.jpg)
All lanes : Anti-Transportin 1 antibody [D45] (ab10303) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
IHC image of ab10303 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10303, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HeLa cells stained with ab10303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10303, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/14101_Transportin-1-Primary-antibodies-ab10303-9.jpg)
Overlay histogram showing HeLa cells stained with ab10303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10303, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab10303 staining cultured P0 mouse neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% Triton X for 30 minutes at 24°C. The cultured neurons were then stained with ab10303 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µMSee Abreview
ICC/IF image of ab10303 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified - Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified
Yu SH, Boyce M, Wands AM, Bond MR, Bertozzi CR, Kohler JJ. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4834-9. doi:
Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel - Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel
Gu L, Tsuji T, Jarboui MA, Yeo GP, Sheehy N, Hall WW, Gautier VW. Retrovirology. 2011 Mar 14;8:17.
Transportin1: a marker of FTLD-FUS. - Transportin1: a marker of FTLD-FUS.
Brelstaff J, Lashley T, Holton JL, Lees AJ, Rossor MN, Bandopadhyay R, Revesz T. Acta Neuropathol. 2011 Nov;122(5):591-600.
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone [corrected] induces CRM1-dependent - 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone [corrected] induces CRM1-dependent
Chen L, Shao C, Cobos E, Wang JS, Gao W. Toxicol Sci. 2010 Jul;116(1):206-15.
Transportin regulates nuclear import of CD44. - Transportin regulates nuclear import of CD44.
Janiszewska M, De Vito C, Le Bitoux MA, Fusco C, Stamenkovic I. J Biol Chem. 2010 Oct 1;285(40):30548-57.
Chemokine receptor CCR2 undergoes transportin1-dependent nuclear translocation. - Chemokine receptor CCR2 undergoes transportin1-dependent nuclear translocation.
Favre N, Camps M, Arod C, Chabert C, Rommel C, Pasquali C. Proteomics. 2008 Nov;8(21):4560-76.