Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180947 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TRBP with ab180947 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG Isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling TRBP with ab180947 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). DAPI staining (blue).
Immunohistochemical analysis of paraffin-embedded Human Infiltrating duct carcinoma of breast tissue labeling TRBP with ab180947 at 1/250 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-TRBP antibody [EPR13550] (ab180947) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution