Anti-TRF1 antibody [TRF-78]
| Name | Anti-TRF1 antibody [TRF-78] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10579 |
| Prices | $395.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | TRF-78 |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Human TRF1 protein produce in baculovirus |
| Description | Mouse Monoclonal |
| Gene | TERF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody. A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation. The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain.
ab10579 at 1/500 staining human HeLa cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 555 conjugated donkey anti-mouse antibody was used as the secondary. See Abreview
Product References
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SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and - SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and
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Acacetin and chrysin, two polyphenolic compounds, alleviate telomeric position - Acacetin and chrysin, two polyphenolic compounds, alleviate telomeric position
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Essential role for mammalian apurinic/apyrimidinic (AP) endonuclease Ape1/Ref-1 - Essential role for mammalian apurinic/apyrimidinic (AP) endonuclease Ape1/Ref-1
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RECQL4, the protein mutated in Rothmund-Thomson syndrome, functions in telomere - RECQL4, the protein mutated in Rothmund-Thomson syndrome, functions in telomere
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53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of
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Telomeric damage in early stage of chronic lymphocytic leukemia correlates with - Telomeric damage in early stage of chronic lymphocytic leukemia correlates with
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