TERF1

Gene Symbol TERF1
Entrez Gene 7013
Alt Symbol PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1
Species Human
Gene Type protein-coding
Description telomeric repeat binding factor (NIMA-interacting) 1
Other Description NIMA-interacting protein 2|TTAGGG repeat-binding factor 1|telomeric protein Pin2/TRF1|telomeric repeat-binding factor 1
Swissprots Q93029 Q15553 Q8NHT6 A7XP29 P54274
Accessions ABV02580 EAW86990 EAW86991 EAW86992 EAW86993 EAW86994 P54274 AF003001 AAB81137 AI218408 AK123410 AK292529 BAF85218 AY576778 AAT81150 BC014646 BC029378 AAH29378 BC071975 BF056993 CX788828 DA658766 DQ892573 ABM83499 DQ895789 ABM86715 U40705 AAB54036 U74382 AAB53363 X93511 CAA63768 XM_005251291 XP_005251348 XM_005251292 XP_005251349 XM_011517582 XP_011515884 NM_003218 NP_003209 NM_017489 NP_059523
Function Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double- stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. {ECO:0000269|PubMed:16166375}.
Subcellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Note=Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
Tissue Specificity Highly expressed and ubiquitous. Isoform Pin2 predominates.

Trf1 (BED5) Mouse mAb - 3529 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone BED5
Western blot analysis of extracts from COS, Jurkat and K-562 cells using Trf1 (BED5) Mouse mAb.

TRF1 (G-7) - sc-271485 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TRF1 (G-7): sc-271485. Western blot analysis of TRF1 expression in Jurkat whole cell lysate.

TRF1 (N-19)-R - sc-6165-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of full length and C-terminally deleted human recombinant fusion protein (A,B). Antibodies tested include TRF1 (C-19): sc-1977 (A) and TRF1 (N-19): sc-6165 (B).

TRF1 (C-19) - sc-1977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of full length and C-terminally deleted human recombinant TRF1 fusion protein (A,B). Antibodies tested include TRF1 (C-19): sc-1977 (A) and TRF1 (N-19): sc-6165 (B).

TRF1 (H-242) - sc-5596 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TRF1 (H-242): sc-5596. Western blot analysis of TRF1 expression in Jurkat whole cell lysate.

TRF1 (TRF-78) - sc-56807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TRF1 (TRF-78): sc-56807. Western blot analysis of TRF1 expression in HeLa whole cell lysate.

Anti-TRF1 (phospho S219) antibody - ab79187 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-TRF1 (phospho S219) antibody (ab79187) at 1/500 dilutionLane 3 : A different TRF1 antibody (ab63374)Lane 1 : 293 cells treated with paclitaxel (1 µM, 24 hours)Lane 2 : 293 cells treated with paclitaxel (1 µM, 24 hours) with immunizing phosphopeptide at 10 µgLane 3 : 293 cells treated with paclitaxel (1 µM, 24 hours)Lysates/proteins at 30 µg per lane.

Anti-TRF1 antibody - ab197661 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TRF1 antibody (ab197661) at 1/400 dilution + Human fetal kidney tissue lysate at 50 µg

Anti-TRF1 antibody - ab167665 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-TRF1 antibody (ab167665) at 1 µg/mlLane 1 : TRF1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-TRF1 antibody - ab71443 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-TRF1 antibody (ab71443) at 1/60 dilution + Jurkat cell lysate at 35 µg

Anti-TRF1 antibody - ab85871 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRF1 antibody (ab85871) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TRF1 antibody - ab63374 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRF1 antibody (ab63374) at 1/500 dilutionLane 1 : 293 cell extract, treated with Paclitaxel (1 micromolar, 24 hours)Lane 2 : 293 cell extract, treated with Paclitaxel (1 micromolar, 24 hours) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-TRF1 antibody - ab2447 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab2447 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence

Anti-TRF1 antibody - ab111154 from Abcam

Host Goat
Clonality Polyclonal
ab111154, at 3.75 µg/ml, staining TRF1 in formalin fixed, paraffin embedded Human Skeletal muscle tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.

Anti-TRF1 antibody - ab13792 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TRF1 antibody [3H11] - ab14397 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3H11
ICC/IF image of ab14397 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14397, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TRF1 antibody [57-6] - ab66223 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 57-6
Anti-TRF1 antibody [57-6] (ab66223) at 0.5 µg/ml + HeLa whole cell extract at 25 µg

Anti-TRF1 antibody [BED5] - ab124273 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone BED5
ab124273, at 10 µg/ml, staining TRF1 in formalin fixed, paraffin embedded Human uterus tissue by Immunohistochemistry.

Anti-TRF1 antibody [BED5 57-6] - BSA and Azide free - ab175241 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BED5 57-6

Anti-TRF1 antibody [EPR6313] - ab129177 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6313
All lanes : Anti-TRF1 antibody [EPR6313] (ab129177) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : Caco 2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-TRF1 antibody [EPR6313] (HRP) - ab198968 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6313
All lanes : Anti-TRF1 antibody [EPR6313] (HRP) (ab198968) at 1/5000 dilutionLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-TRF1 antibody [TRF-78] - ab10579 from Abcam

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone TRF-78
Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody.  A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation.  The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain. 

Anti-TRF1 antibody - C-terminal - ab137690 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TRF1 antibody - C-terminal (ab137690) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from A431 cellsLane 2 : Whole cell lysate prepared from H1299 cellsLane 3 : Whole cell lysate prepared from HeLa cellsLane 4 : Whole cell lysate prepared from HepG2 cellsLysates/proteins at 30 µg per lane.

Anti-TRF1 antibody - ChIP Grade - ab1423 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.

Anti-TRF1 / TERF1 Antibody - LS-C335079 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of 293 cell line, using TERF1 antibody.
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