All lanes : Anti-TRF2 antibody (ab4182) at 1 µg/mlLane 1 : HeLa nuclear extractLane 2 : HeLa whole cell lysateLane 3 : HeLa nuclear lysate with Human TRF2 peptide (ab13765) at 1 µg/mlLane 4 : HeLa whole cell lysate with Human TRF2 peptide (ab13765) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
ICC/IF image of ab4182 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab4182, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab4182 staining TRF2 in human Breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton-x100 and blocking with 2% serum was performed for 30 minutes at 370C. Samples were incubated with primary antibody at 1/100 dilution in PBS for 2 hours at 37°C. An Alexa Fluor® 594 conjugated goat monoclonal to rabbit IgG was used at 1/75 dilution as secondary antibody.See Abreview