Anti-TRF2 antibody [4A794] - ChIP Grade

• Supplier: Abcam
• Catalog: ab13579
• Prices: $394.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 4A794
• Applications: IHC-P WB ICC/IF IP ChIP ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human, Deer
• Antigen: Baculovirus expressing full length TRF2 protein
• Description: Mouse Monoclonal
• Gene: TERF2
• Conjugate: Unconjugated

Product images

ab13579 staining TRF2 in bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579.  2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein. Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ab13579 staining TRF2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.The cells were washed in PBS, fixed for 15 minutes at room temperature in 4% formaldehyde in PBS, and then washed for 5 minutes with PBS. The cells were then dehydrated with cold methanol at room temperature for 10 minutes, washed, and blocked with blocking solution (3% skim milk, 0.05% Tween-20 in PBS) at room temperature for 1 hour. ab13579 added at 2 µg/ml at 37?°C for 1 hour. After washing three times, 5 µg/mL fluorescein (TRITC) goat anti-mouse (Abcam) as the secondary antibody in blocking solution was added and incubated at room temperature for 1 hour.

Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to - Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to

Yuan P, Wang Z, Lv W, Pan H, Yang Y, Yuan X, Hu J. Oncol Lett. 2014 Sep;8(3):1009-1016. Epub 2014 Jul 2.

Lamin B1 fluctuations have differential effects on cellular proliferation and - Lamin B1 fluctuations have differential effects on cellular proliferation and

Dreesen O, Chojnowski A, Ong PF, Zhao TY, Common JE, Lunny D, Lane EB, Lee SJ, Vardy LA, Stewart CL, Colman A. J Cell Biol. 2013 Mar 4;200(5):605-17.

Telomeric repeat-containing RNA and telomerase in human fetal oocytes. - Telomeric repeat-containing RNA and telomerase in human fetal oocytes.

Reig-Viader R, Brieno-Enriquez MA, Khouriauli L, Toran N, Cabero L, Giulotto E, Garcia-Caldes M, Ruiz-Herrera A. Hum Reprod. 2013 Feb;28(2):414-22.

53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of

Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grofte M, Chan KL, Hickson ID, Bartek J, Lukas J. Nat Cell Biol. 2011 Mar;13(3):243-53.

TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric - TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric

Bombarde O, Boby C, Gomez D, Frit P, Giraud-Panis MJ, Gilson E, Salles B, Calsou P. EMBO J. 2010 May 5;29(9):1573-84.

Telomeric repeat-containing RNA structure in living cells. - Telomeric repeat-containing RNA structure in living cells.

Xu Y, Suzuki Y, Ito K, Komiyama M. Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14579-84. doi:

Purification of proteins associated with specific genomic Loci. - Purification of proteins associated with specific genomic Loci.

Dejardin J, Kingston RE. Cell. 2009 Jan 9;136(1):175-86.

Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of - Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of

Deng Z, Lezina L, Chen CJ, Shtivelband S, So W, Lieberman PM. Mol Cell. 2002 Mar;9(3):493-503.

Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom - Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom

Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA. J Biol Chem. 2002 Oct 25;277(43):41110-9. Epub 2002 Aug 13.

The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere - The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere

Johnson FB, Marciniak RA, McVey M, Stewart SA, Hahn WC, Guarente L. EMBO J. 2001 Feb 15;20(4):905-13.