Anti-TRIF antibody
| Name | Anti-TRIF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab13810 |
| Prices | $369.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide, corresponding to amino acids 143-158 of Mouse TRIF |
| Description | Rabbit Polyclonal |
| Gene | TICAM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot analysis of TICAM in mouse spleen lysate using ab13810 at 1:1000 dilution.
ab13810 (2µg/ml) staining TRIF in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of mucosal cell cytoplasm.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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