All lanes : Anti-TrkA (phospho Y490) antibody (ab85130) at 1 µg/mlLane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Human TrkA (phospho Y490) peptide (ab99483) at 1 µg/mlLane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with TrkA peptide (ab116686) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab85130 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85130 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.