Anti-TrkA antibody
| Name | Anti-TrkA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8871 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP WB ICC/IF IHC-F |
| Species Reactivities | Rat |
| Antigen | Extracellular fragment from the rat Trk A receptor (amino acids 1-416) |
| Description | Rabbit Polyclonal |
| Gene | Ntrk1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining for TrkA obtained with TrkA antibody (ab8871) in rat DRG. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : IHC on 30um cryostat sections. Primary antibody ab8871 was used at 1/3000 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.
All lanes : Anti-TrkA antibody (ab8871) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from rat PC12 cells, un-treated.Lane 2 : Whole cell lysate prepared from rat PC12 cells, treated with 10 ng/ml NGF for 5 minutes.Lysates/proteins at 200000 cells per lane.SecondaryHRP Goat anti-rabbit at 1/10000 dilutionObserved band size : 110,140 kDa (why is the actual band size different from the predicted?)Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.Image courtesy of an Abreview submitted by Joe Makkerh.
Product References
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Autoantibodies to neurotrophic receptors TrkA, TrkB and TrkC in patients with - Autoantibodies to neurotrophic receptors TrkA, TrkB and TrkC in patients with
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