ab75174 at 1/500 dilution stained rat DRG, DAPI counterstained, using Immunofluorescence
All lanes : Anti-TrkC antibody (ab75174) at 1/1000 dilutionLane 1 : Conrtol: TrkC proteinLane 2 : Human brain lysateLane 3 : rat brain lysateLane 4 : Rat brain lysate Lane 5 : Mouse brain lysateLysates/proteins at 30 µg per lane.SecondaryGoat anti-rabbit IgG HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab75174 staining TrkC in the NIH/3T3 cells (mouse Fibroblasts)cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with Saponin 0.1% in PBS and blocked with 4% Serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 4% Serum) for 16 hours at 4°C. An FITC-conjugated Goat anti-rabbit IgG polyclonal(1/100) was used as the secondary antibody.See Abreview