Anti-TRP1 antibody [G3E6]
| Name | Anti-TRP1 antibody [G3E6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49684 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | G3E6 |
| Applications | ICC/IF ICC/IF IHC-P |
| Species Reactivities | Dog, Human |
| Antigen | Recombinant fusion protein corresponding to the C terminus of human TRP1 |
| Description | Mouse Monoclonal |
| Gene | TYRP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab49684 staining TRP1 in Dog skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate, pH 6.0. Samples were incubated with primary antibody (1/50 in PBS + 1x casein) for 1 hour at 37°C. An undiluted HRP-conjugated Hydra vulgaris anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
ICC/IF image of ab49684 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49684, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Human malignant melanoma: IHC-P staining for tyrosinase-related protein-1 using ab49684. Cytoplasmic staining of tumor cells.
Product References
TYRP1 mRNA expression in melanoma metastases correlates with clinical outcome. - TYRP1 mRNA expression in melanoma metastases correlates with clinical outcome.
Journe F, Id Boufker H, Van Kempen L, Galibert MD, Wiedig M, Sales F, Theunis A, Nonclercq D, Frau A, Laurent G, Awada A, Ghanem G. Br J Cancer. 2011 Nov 22;105(11):1726-32.
Absence of TRP-2 in melanogenic melanocytes of human hair. - Absence of TRP-2 in melanogenic melanocytes of human hair.
Commo S, Gaillard O, Thibaut S, Bernard BA. Pigment Cell Res. 2004 Oct;17(5):488-97.