Anti-TRPM7 antibody [S74-25]
| Name | Anti-TRPM7 antibody [S74-25] |
|---|---|
| Supplier | Abcam |
| Catalog | ab85016 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S74-25 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Fusion protein: LKLPDLKRND YTPDKIIFPQ DESSDLNLQS GNSTKESEAT NSVRLML, corresponding to cytoplasmic C terminal amino acids 1817-1863 of mouse TRPM7 |
| Description | Mouse Monoclonal |
| Gene | TRPM7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab85016 staining TRPM7 in cultured mouse urothelial cells by Immunocytochemistry. Note that TRPM7 (green) is present in the plasma membrane as well as cytoplasmic vesicles. Nuclei stained blue with DAPI.Urothelial cells from mice were fixed with 3.7% formaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 for 10 minutes, and blocked with 3% BSA for 3 hours. The primary antibody was used at a 1/400 dilution and incubated overnight. The secondary antibody was an Alexa Fluor488-conjugated anti-rabbit IgG, used at a 1/1000 dilution, applied for 1 hour at room temperature. Triple washing with PBS was performed between each step.
![All lanes : Anti-TRPM7 antibody [S74-25] (ab85016) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/15323_TRPM7-Primary-antibodies-ab85016-1.jpg)
All lanes : Anti-TRPM7 antibody [S74-25] (ab85016) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg
ab85016 staining TRPM7 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
![Overlay histogram showing HeLa cells stained with ab85016 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85016, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/15325_TRPM7-Primary-antibodies-ab85016-4.jpg)
Overlay histogram showing HeLa cells stained with ab85016 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85016, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic - Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic
Zhu Y, Men R, Wen M, Hu X, Liu X, Yang L. Life Sci. 2014 Jan 14;94(1):37-44.
TRPM7 channels regulate glioma stem cell through STAT3 and Notch signaling - TRPM7 channels regulate glioma stem cell through STAT3 and Notch signaling
Liu M, Inoue K, Leng T, Guo S, Xiong ZG. Cell Signal. 2014 Dec;26(12):2773-81.
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial - Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial
Sun H, Leng T, Zeng Z, Gao X, Inoue K, Xiong ZG. PLoS One. 2013 Nov 1;8(11):e79540.
Functional characterization of transient receptor potential channels in mouse - Functional characterization of transient receptor potential channels in mouse
Everaerts W, Vriens J, Owsianik G, Appendino G, Voets T, De Ridder D, Nilius B. Am J Physiol Renal Physiol. 2010 Mar;298(3):F692-701. doi: