Anti-TRPS1 antibody
| Name | Anti-TRPS1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab125197 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Chimpanzee, Monkey, Ape, Orangutan, Platypus |
| Antigen | Synthetic peptide, corresponding to a region within amino acids 1000-1050 of Human TRPS1 (AAG21134 |
| Description | Rabbit Polyclonal |
| Gene | TRPS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TRPS1 antibody (ab125197) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
ab125197 at 1 µg/ml staining TRPS1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells. Lane 1; IP using ab125197 at 6 µg/mg lysate. Lane 2; IP using control IgG. 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 30 second exposure.
All lanes : Anti-TRPS1 antibody (ab125197) at 1/500 dilutionLane 1 : Ha-Ras-transformed EpH4 cells - nuclear extractsLane 2 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extractsLane 3 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extractsLane 4 : EpH4 (parental) - nuclear extractsLane 5 : EpF-2 - nuclear extractsLane 6 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extractsLane 7 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extractsLane 8 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extractsLane 9 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extractsLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilutionPerformed under reducing conditions.