Anti-TRPV4 antibody
| Name | Anti-TRPV4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39260 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F WB IHC-P |
| Species Reactivities | Mouse, Rat, Horse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse TRPV4 |
| Blocking Peptide | Mouse TRPV4 peptide |
| Description | Rabbit Polyclonal |
| Gene | TRPV4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab39260 staining of TRPV4 -/- (upper panel) and wildtype (lower panel) adult mouse kidney tissue sections, showing good immunostaining in the wildtype tissue and no immunostaining in the TRPV4 -/- tissue. Formalin/PFA-fixed paraffin-embedded sections of mouse kidney tissue were incubated with ab39260 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0. A biotin-conjugated goat anti-rabbit antibody was used as the secondary.See Abreview
ICC/IF image of ab39260 stained PC12 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab39260, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-TRPV4 antibody (ab39260) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 25 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-FoFr image of TRPV4 staining on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat. The image shows DRG neurons (left), nerve taken at the level of the root (right) See Abreview
All lanes : Anti-TRPV4 antibody (ab39260) at 1 µg/mlLane 1 : Whole cell lysate prepared from MEF cellsLane 2 : Whole cell lysate prepared from F9 cellsLane 3 : Whole cell lysate prepared from GC-1 cellsLane 4 : Whole cell lysate prepared from NIH-3T3 cellsLysates/proteins at 20 µg per lane.SecondaryHRP conjugated pig anti-rabbit polyclonal at 1/5000 dilutiondeveloped using the ECL technique
Product References
Effects of cyclic equibiaxial mechanical stretch on alpha-BK and TRPV4 expression - Effects of cyclic equibiaxial mechanical stretch on alpha-BK and TRPV4 expression
Hdud IM, Mobasheri A, Loughna PT. Springerplus. 2014 Jan 29;3:59.
Thyroid hormone is required for hypothalamic neurons regulating cardiovascular - Thyroid hormone is required for hypothalamic neurons regulating cardiovascular
Mittag J, Lyons DJ, Sallstrom J, Vujovic M, Dudazy-Gralla S, Warner A, Wallis K, Alkemade A, Nordstrom K, Monyer H, Broberger C, Arner A, Vennstrom B. J Clin Invest. 2013 Jan;123(1):509-16.
Transient receptor potential vanilloid-2 mediates the effects of transient heat - Transient receptor potential vanilloid-2 mediates the effects of transient heat
Szollosi AG, Olah A, Toth IB, Papp F, Czifra G, Panyi G, Biro T. FEBS Lett. 2013 May 2;587(9):1440-5.
UVB radiation generates sunburn pain and affects skin by activating epidermal - UVB radiation generates sunburn pain and affects skin by activating epidermal
Moore C, Cevikbas F, Pasolli HA, Chen Y, Kong W, Kempkes C, Parekh P, Lee SH, Kontchou NA, Yeh I, Jokerst NM, Fuchs E, Steinhoff M, Liedtke WB. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3225-34. doi:
Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different - Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different
Hdud IM, El-Shafei AA, Loughna P, Barrett-Jolley R, Mobasheri A. Int J Mol Sci. 2012;13(4):4433-45.
TRPV4 channels mediate the infrared laser-evoked response in sensory neurons. - TRPV4 channels mediate the infrared laser-evoked response in sensory neurons.
Albert ES, Bec JM, Desmadryl G, Chekroud K, Travo C, Gaboyard S, Bardin F, Marc I, Dumas M, Lenaers G, Hamel C, Muller A, Chabbert C. J Neurophysiol. 2012 Jun;107(12):3227-34.
The molecular and cellular identity of peripheral osmoreceptors. - The molecular and cellular identity of peripheral osmoreceptors.
Lechner SG, Markworth S, Poole K, Smith ES, Lapatsina L, Frahm S, May M, Pischke S, Suzuki M, Ibanez-Tallon I, Luft FC, Jordan J, Lewin GR. Neuron. 2011 Jan 27;69(2):332-44.
Mutations in TRPV4 cause an inherited arthropathy of hands and feet. - Mutations in TRPV4 cause an inherited arthropathy of hands and feet.
Lamande SR, Yuan Y, Gresshoff IL, Rowley L, Belluoccio D, Kaluarachchi K, Little CB, Botzenhart E, Zerres K, Amor DJ, Cole WG, Savarirayan R, McIntyre P, Bateman JF. Nat Genet. 2011 Oct 2;43(11):1142-6.