Overlay histogram showing HEK293 cells stained with ab137028 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137028, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-TRPV5 antibody [EPR8875] (ab137028) at 1/10000 dilutionLane 1 : Fetal kidney lysateLane 2 : Human small intestine lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLane 5 : Saos-2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling TRPV5 with ab137028 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TRPV5 with ab137028 at 1/100 dilution.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD