Anti-TSG101 antibody [4A10]

• Supplier: Abcam
• Catalog: ab83
• Prices: $401.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 4A10
• Applications: FC ICC/IF ICC/IF IP WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Fusion protein containing amino acids 167-374 of the TSG101 protein expressed in E
• Description: Mouse Monoclonal
• Gene: TSG101
• Conjugate: Unconjugated

Product images

All lanes : Anti-TSG101 antibody [4A10] (ab83)Lane 1 : 20ug HeLa cell extractLane 2 : 20ug HeLa cell extract from cells treated with TSG101 siRNA

All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryDonkey polyclonal to Mouse IgG (IRDyeTM 700DX) at 1/10000 dilution

Immunofluorescence analysis of amyloid peptide-treated mouse astrocytes, staining TSG101 with ab83. Astrocytes were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 for 5 min at room temperature. Cells were incubated with primary antibody and a fluorescent-conjugated anti-mouse IgG was used as the secondary antibody.

Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.

Product References

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