Cyclin B1 (D5C10) XP ® Rabbit mAb
| Name | Cyclin B1 (D5C10) XP ® Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12231 |
| Prices | $109.00, $277.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D5C10 |
| Applications | WB IP ICC/IF FC |
| Species Reactivities | Human, Rat |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cyclin B1 protein. |
| Description | Rabbit Monoclonal |
| Gene | CCNB1 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Cyclin B1 (D5C10) XP ® Rabbit mAb.
Western blot analysis of extracts from HT-29 cells, synchronized in S-phase by double thymidine block (2 nM, 16 hr) followed by release into fresh media for the indicated time, using Cyclin B1 (D5C10) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of HT-29 cells using Cyclin B1 (D5C10) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Cyclin B1 (D5C10) XP ® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 (DNA content). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.