Anti-TSH Receptor antibody [A7]
| Name | Anti-TSH Receptor antibody [A7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6044 |
| Prices | $381.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | A7 |
| Applications | IHC-F WB FC ELISA IP ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Fusion protein, corresponding to amino acids 402-415 of Human TSH receptor |
| Description | Mouse Monoclonal |
| Gene | TSHR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab6044 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6044, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab6044 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/15602_TSH-Receptor-Primary-antibodies-ab6044-2.jpg)
Overlay histogram showing HeLa cells stained with ab6044 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Direct binding of thyrotropin receptor autoantibody to in vitro translated - Direct binding of thyrotropin receptor autoantibody to in vitro translated
Morgenthaler NG, Hodak K, Seissler J, Steinbrenner H, Pampel I, Gupta M, McGregor AM, Scherbaum WA, Banga JP. Thyroid. 1999 May;9(5):466-75.
Monoclonal antibodies to the human TSH receptor: epitope mapping and binding to - Monoclonal antibodies to the human TSH receptor: epitope mapping and binding to
Nicholson LB, Vlase H, Graves P, Nilsson M, Molne J, Huang GC, Morgenthaler NG, Davies TF, McGregor AM, Banga JP. J Mol Endocrinol. 1996 Apr;16(2):159-70.