Anti-TSLP antibody
| Name | Anti-TSLP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47943 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Recombinant full length TSLP protein (Human),>98% pure |
| Description | Rabbit Polyclonal |
| Gene | TSLP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab47943 staining TSLP in human prostate tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
Anti-TSLP antibody (ab47943) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab47943 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The short form of TSLP is constitutively translated in human keratinocytes and - The short form of TSLP is constitutively translated in human keratinocytes and
Bjerkan L, Schreurs O, Engen SA, Jahnsen FL, Baekkevold ES, Blix IJ, Schenck K. Mucosal Immunol. 2015 Jan;8(1):49-56.
The 420K LEKTI variant alters LEKTI proteolytic activation and results in - The 420K LEKTI variant alters LEKTI proteolytic activation and results in
Fortugno P, Furio L, Teson M, Berretti M, El Hachem M, Zambruno G, Hovnanian A, D'Alessio M. Hum Mol Genet. 2012 Oct 1;21(19):4187-200.
Thymic stromal lymphopoietin is a key mediator of breast cancer progression. - Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
Olkhanud PB, Rochman Y, Bodogai M, Malchinkhuu E, Wejksza K, Xu M, Gress RE, Hesdorffer C, Leonard WJ, Biragyn A. J Immunol. 2011 May 15;186(10):5656-62.