Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution + MDA-MB-435 at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution + Human testis at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling TSP50 with ab181993 at a 1/150 dilution, or with a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.