PRSS50

Gene Symbol PRSS50
Entrez Gene 29122
Alt Symbol CT20, TSP50
Species Human
Gene Type protein-coding
Description protease, serine, 50
Other Description cancer/testis antigen 20|probable threonine protease PRSS50|serine protease 50|testes specific protease 50|testes-specific protease 50|testis-specific protease-like protein 50
Swissprots Q9UI38
Accessions CAI30701 EAW64782 Q9UI38 AF100707 AAF22500 BC033016 AAH33016 BC037775 AAH37775 EU176497 ABW03298 NM_013270 NP_037402
Function May be involved in proteolysis through its threonine endopeptidase activity. {ECO:0000269|PubMed:17283160}.
Subcellular Location Endoplasmic reticulum {ECO:0000305|PubMed:17283160}. Note=May also localize to cytoplasmic membranes.
Tissue Specificity Testis specific. Differentially expressed in some breast cancer tissues.

PRSS50 (N-17) - sc-49559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PRSS50 (T-19) - sc-49561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRSS50 (T-19): sc-49561. Western blot analysis of PRSS50 expression in HeLa (A), MCF7 (B), HEK293 (C), K-562 (D) and T-47D (E) whole cell lysates.

Anti-TSP50 antibody - ab168993 from Abcam

Host Mouse
Clonality Polyclonal
All lanes : Anti-TSP50 antibody (ab168993) at 1/500 dilutionLane 1 : TSP50 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-TSP50 antibody - ab59789 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TSP50 antibody [EPR8675(2)] - ab181993 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8675(2)
Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution + MDA-MB-435 at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PRSS50 / TSP50 Antibody (N-Terminus) - LS-C144698 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TSP50 Antibody - 17190002 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: TSP50 Antibody [17190002] - Samples: Lane 1, Marker [kDa]: 219, 111, 83, 48, 32, 26, 17  Lane 2, RT-4  Lane 3, U-251MG sp  Lane 4, Human Plasma  Lane 5, Liver  Lane 6, Tonsil,  Target weight [kDa]:   43   Validation score:   2   Validation description:  Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

TSP50 Antibody [Unconjugated] - AF2430 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western  blot shows lysates of human testis tissue. PVDF membrane was probed with  0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified  Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat  IgG Secondary Antibody (Catalog # HAF017).  A specific band was detected for TSP50 at approximately 55 kDa (as  indicated). This experiment was conducted under reducing conditions and using  Immunoblot  Buffer Group 1.

TSP50 Antibody (260606) - MAB2430 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Rat
Clonality Monoclonal
Clone 260606

TSP50 Antibody - NBP1-19027 from Novus Biologicals

Prices $329.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

TSP50 Antibody - NBP2-34204 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: TSP50 Antibody [NBP2-34204] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

TSP50 Antibody - H00029122-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: TSP50 Antibody [H00029122-B01] - analysis of TSP50 expression in transfected 293T cell line by TSP50 MaxPab polyclonal antibody (B01). Lane 1: TSP50 transfected lysate (43.10 KDa). Lane 2: Non-transfected lysate.

TSP50 Antibody [Biotin] - BAF2430 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

TSP50 Antibody (2D8) - H00029122-M10 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D8
Sandwich ELISA: TSP50 Antibody (2D8) [H00029122-M10] - Detection limit for recombinant GST tagged TSP50 is 0.3 ng/ml as a capture antibody.

TSP50 Polyclonal Antibody - bs-6745R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TSP50 (88-100) antibody produced in rabbit - SAB1101242 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-TSP50 (88-100) (RB): <B>Cat. No. SAB1101242</B>: Immunofluorescence of HUVEC cells using TSP50 (88-100) (RB), Cat. No. SAB1101242 (red) at a 1:50 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-PRSS50 antibody produced in rabbit - HPA040768 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PRSS50: <B>Cat. No. HPA040768</B>: Immunohistochemical staining of Human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Human TSP50 Antibody - AF2430 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Detection of Human TSP50 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Human TSP50 Affinity Purified Polyclonal Ab, Goat IgG - AF2430-SP from R&D Systems

Host Goat
Clonality Polyclonal

Human TSP50 Biotinylated Antibody - BAF2430 from R&D Systems

Prices $390.00
Sizes 50 µg
Host Goat

Human TSP50 Antibody - MAB2430 from R&D Systems

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Rat
Clone 260606

Human TSP50 MAb (Clone 260606), Rat IgG2A - MAB2430-SP from R&D Systems

Host Rat
Clonality Monoclonal
Clone 260606

Mouse TSP50 Antibody - AF2455 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat

Mouse TSP50 Affinity Purified Polyclonal Ab, Goat IgG - AF2455-SP from R&D Systems

Host Goat
Clonality Polyclonal

Mouse TSP50 Antibody - MAB2455 from R&D Systems

Prices $99.00, $339.00
Sizes 25 µg, 500 µg
Host Rat
Clone 260514
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