Anti-TTF1 antibody [EP1584Y]

• Supplier: Abcam
• Catalog: ab76013
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1584Y
• Applications: ICC/IF ICC/IF IHC-P WB ICC/IF FC IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues near the N-terminus of human TTF1
• Blocking Peptide: TTF1 peptide
• Description: Rabbit Monoclonal
• Gene: NKX2-1
• Conjugate: Unconjugated

Product images

Overlay histogram showing A549 cells stained with ab76013 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76013, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : Mouse lung lysateLane 3 : Rat lung lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilutiondeveloped using the ECL technique

ab76013, at 1/100 dilution, staining TTF1 in human thyroid carcinoma by immunohistochemistry using paraffin-embedded tissue.

ab76013, at 1/100 dilution, staining TTF1 in human lung adenocarcinoma by immunohistochemistry using paraffin-embedded tissue.

ab76013 staining TTF1 in murine fetal lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde and permeabilized using PBST. Samples were then blocked using 1.5% serum for 20 minutes at 25°C, then incubated with ab76013 at a 1/400 dilution for 12 hours at 4°C. A biotin conjugated goat anti-rabbit IgG was used as the secondary at a 1/200 dilution.See Abreview

Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution + HEK293 whole cell lysate at 30 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Immunofluorescence analysis of rat bronchi cells, staining TTF1 with ab76013. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. See Abreview

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