NKX2-1

Gene Symbol NKX2-1
Entrez Gene 7080
Alt Symbol BCH, BHC, NK-2, NKX2.1, NKX2A, T/EBP, TEBP, TITF1, TTF-1, TTF1
Species Human
Gene Type protein-coding
Description NK2 homeobox 1
Other Description NK-2 homolog A|homeobox protein NK-2 homolog A|homeobox protein Nkx-2.1|thyroid nuclear factor 1|thyroid transcription factor 1|thyroid-specific enhancer-binding protein
Swissprots P43699 O14954 Q9BRJ8 Q7KZF6 O14955 D3DSA3
Accessions AAA83233 AAC12924 AAC50125 BAA23527 BAB56137 EAW65856 EAW65857 EAW65858 EAW65859 P43699 BC006221 AAH06221 BE796718 BM970287 BM970838 BT009773 AAP88775 D50740 BAA23529 D50741 BAA23528 DQ892964 ABM83890 DQ896211 ABM87210 U19756 AAA86099 U33749 AAB52381 U43203 AAA89066 X82850 CAA58053 NM_001079668 NP_001073136 NM_003317 NP_003308
Function Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity). {ECO:0000250|UniProtKB:P23441, ECO:0000250|UniProtKB:P50220}.
Subcellular Location Nucleus {ECO:0000250|UniProtKB:P50220}.
Tissue Specificity Thyroid and lung.

Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb - 12373 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2E8
Western blot analysis of extracts from TTF-1 positive H441 and TT cells, and TTF-1 negative A549 and HeLa cells, using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-TTF-1 (Ser327) Antibody - 13608 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Phospho-TTF-1 (Ser327) Antibody (upper) and β-Actin (D8A7) Rabbit mAb #8457 (lower).

TTF-1 (8G7G3/1) - sc-53136 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TTF-1 (8G7G3/1): sc-53136. Western blot analysis of TTF-1 expression in TT whole cell lysate.

TTF-1 (F-12) - sc-25331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TTF-1 (F-12): sc-25331. Western blot analysis of TTF-1 expression in TT (A) and AMJ2-C11 (B) whole cell lysates.

TTF-1 (C-20) - sc-8762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TTF-1 (H-190) - sc-13040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TTF-1 (H-190): sc-13040. Immunofluorescence staining of methanol-fixed TT cells showing nuclear localization.

TTF-1 (N-19) - sc-8761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TTF-1 (N-19): sc-8761. Western blot analysis of TTF-1 expression in non-transfected: sc-117752 (A) and human TTF-1 transfected: sc-159187 (B) 293T whole cell lysates.

TTF-1 (G-17) - sc-12524 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TTF-1 (SPM150) - sc-56606 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TTF-1 (SPM150): sc-56606. Western blot analysis of TTF-1 expression in TT whole cell lysate.

Anti-TTF1 antibody - ab125650 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of TTF1 in Human lung tissue with ab125650 at 20 ug/mL.

Anti-TTF1 antibody - ab86023 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TTF1 antibody (ab86023) at 1/1000 dilutionLane 1 : An uninduced (negative control) culture of E.coliLane 2 : An induced culture of E.coliSecondaryAnti-Rabbit IgG HRP at 1/1000 dilution

Anti-TTF1 antibody - ab173517 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TTF1 antibody (ab173517) at 1/100 dilution + ZR-75-1 cell line lysate at 35 µg

Anti-TTF1 antibody [8G7G3/1] - ab72876 from Abcam

Prices $382.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 8G7G3/1
ab72876 staining TTF1 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/50 in 10% goat serum in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-TTF1 antibody [8G7G3/1] - ab140245 from Abcam

Prices $378.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 8G7G3/1
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat lung tissue labelling TTF1 with ab140245.

Anti-TTF1 antibody [8G7G3/1] (Biotin) - ab140242 from Abcam

Prices $376.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 8G7G3/1
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat lung tissue labelling TTF1 with ab140242.

Anti-TTF1 antibody [A22-A] - N-terminal - ab179871 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone A22-A
All lanes : Anti-TTF1 antibody [A22-A] - N-terminal (ab179871) at 1/1000 dilutionLane 1 : Cell lysate transient transfected with Human TTF1 at 5 µlLane 2 : Cell lysate transient transfected with Human TTF1 at 15 µl

Anti-TTF1 antibody [EP1584Y] - ab76013 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1584Y
Overlay histogram showing A549 cells stained with ab76013 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76013, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-TTF1 antibody [EP1584Y] (Alexa Fluor® 488) - ab196470 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1584Y
ab196470 staining TTF1 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196470 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2 µg/mL (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TTF1 antibody [EP1584Y] (Alexa Fluor® 647) - ab196517 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1584Y
ab196517 staining TTF-1 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196517 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TTF1 antibody [EPR5955(2)] - ab133737 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5955(2)
All lanes : Anti-TTF1 antibody [EPR5955(2)] (ab133737) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Human fetal lung lysateLane 3 : Rat lung lysateLane 4 : Mouse lung lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-TTF1 antibody [EPR8190] - ab133638 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8190
All lanes : Anti-TTF1 antibody [EPR8190] (ab133638) at 1/1000 dilutionLane 1 : HeLa lysateLane 2 : Human lung adenocarcinoma lysateLane 3 : Fetal lung lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-TTF1 antibody [EPR8190] - ab137061 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8190
Immunohistochemical analysis using ab137061 showing positive staining in Lung squamous carcinoma tissue.

Anti-NKX2-1 / Thyroid-Specific TF Antibody - LS-C10567 from LifeSpan Bioscience

Prices $515.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal

Anti-NKX2-1 / Thyroid-Specific TF Antibody - LS-C10566 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NKX2-1 / Thyroid-Specific TF Antibody (clone 8G7G3/1, Azide-free) - LS-C88379 from LifeSpan Bioscience

Prices $640.00, $955.00
Sizes 100 µg, 200 µg
Host Mouse
Clonality Monoclonal
Clone 8G7G3/1
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