Anti-Twist antibody
| Name | Anti-Twist antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab50581 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | EMSA IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse, Chicken, Chimpanzee |
| Antigen | Synthetic peptide: PADDSLSNSEEEPDRQ conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 12-27 of Human Twist Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | TWIST1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
ab50581 staining Twist in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid buffer pH 6.1. Samples were then blocked with 0.5% BSA for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/500 dilution for 2 hours. A biotin-conjugated secondary antibody was used at a 1/100 dilution.
ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
Product References
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Expression of the epithelial-mesenchymal transition-related proteins and their - Expression of the epithelial-mesenchymal transition-related proteins and their
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Putative prognostic epithelial-to-mesenchymal transition biomarkers for - Putative prognostic epithelial-to-mesenchymal transition biomarkers for
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The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal - The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal
Chen Z, Zhang D, Yue F, Zheng M, Kovacevic Z, Richardson DR. J Biol Chem. 2012 May 18;287(21):17016-28.
Functional cooperation between Snail1 and twist in the regulation of ZEB1 - Functional cooperation between Snail1 and twist in the regulation of ZEB1
Dave N, Guaita-Esteruelas S, Gutarra S, Frias A, Beltran M, Peiro S, de Herreros AG. J Biol Chem. 2011 Apr 8;286(14):12024-32.
Correlation of increased twist with lymph node metastasis in patients with oral - Correlation of increased twist with lymph node metastasis in patients with oral
Wushou A, Pan HY, Liu W, Tian Z, Wang LZ, Shali S, Zhang ZY. J Oral Maxillofac Surg. 2012 Jun;70(6):1473-9.
Hypoxia inhibits osteogenesis in human mesenchymal stem cells through direct - Hypoxia inhibits osteogenesis in human mesenchymal stem cells through direct
Yang DC, Yang MH, Tsai CC, Huang TF, Chen YH, Hung SC. PLoS One. 2011;6(9):e23965.
Cadherin-catenin complex dissociation in lobular neoplasia of the breast. - Cadherin-catenin complex dissociation in lobular neoplasia of the breast.
Morrogh M, Andrade VP, Giri D, Sakr RA, Paik W, Qin LX, Arroyo CD, Brogi E, Morrow M, King TA. Breast Cancer Res Treat. 2012 Apr;132(2):641-52.
Prognostic significance of hypoxia-inducible factor-1alpha, TWIST1 and Snail - Prognostic significance of hypoxia-inducible factor-1alpha, TWIST1 and Snail
Hung JJ, Yang MH, Hsu HS, Hsu WH, Liu JS, Wu KJ. Thorax. 2009 Dec;64(12):1082-9.
Twist is transcriptionally induced by activation of STAT3 and mediates STAT3 - Twist is transcriptionally induced by activation of STAT3 and mediates STAT3
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