All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate
Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein
All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : NIH/3T3 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLane 5 : Raji cell lysateLane 6 : OCM-1 cell lysate
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).