Anti-Twist antibody [Twist2C1a] - ChIP Grade

• Supplier: Abcam
• Catalog: ab50887
• Prices: $403.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: Twist2C1a
• Applications: IHC-P IHC-F IP WB DB ChIP ICC/IF ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment (Human)
• Description: Mouse Monoclonal
• Gene: TWIST1
• Conjugate: Unconjugated

Product images

ChIP analysis using ab50887 binding Twist in Human primary macrophages. Cells were cross-linked for 15 minutes with formaldehyde. Samples were incubated with primary antibody  (1/100) for 12 hours at 4°C. Protein binding was detected using real-time PCR.See Abreview

Ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution

ab50887 staining Twist in Mouse pancreatic cancer (sarcomatoid subtype) tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton X100 (0.025%) and blocked with a MOM kit for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

ab50887 staining Twist in a Rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.See Abreview

ab50887 staining Twist in mouse embryonic mesenchyme tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were then incubated with ab50887 at a 1/60 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse IgG, used at a 1/500 dilution.See Abreview

Lane 1 : Lanes 2 - 5 : Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/50 dilutionLane 1 : Marker laneLane 2 : Hela whole cell lysate, 25µgLane 3 : A2058 whole cell lysate, 25µgLane 4 : NIH3T3 whole cell lysate, 25µgLane 5 : F2408 whole cell lysate, 25µg

Product References

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SRC-1 and Twist1 expression positively correlates with a poor prognosis in human - SRC-1 and Twist1 expression positively correlates with a poor prognosis in human

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Timed deletion of Twist1 in the limb bud reveals age-specific impacts on autopod - Timed deletion of Twist1 in the limb bud reveals age-specific impacts on autopod

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TIMP-1 via TWIST1 induces EMT phenotypes in human breast epithelial cells. - TIMP-1 via TWIST1 induces EMT phenotypes in human breast epithelial cells.

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