Anti-Twist2 antibody
| Name | Anti-Twist2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57997 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-161 of Human Twist2 |
| Description | Mouse Monoclonal |
| Gene | TWIST2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Twist2 antibody (ab57997) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human liver.
Western blot against GST-tagged recombinant protein immunogen using ab57997 Twist2 antibody at 1ug/ml.
ICC/IF image of ab57997 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57997, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab57997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57997, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16511_Twist2-Primary-antibodies-ab57997-2.jpg)
Overlay histogram showing HeLa cells stained with ab57997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57997, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Regulation of p21 by TWIST2 contributes to its tumor-suppressor function in human - Regulation of p21 by TWIST2 contributes to its tumor-suppressor function in human
Zhang X, Ma W, Cui J, Yao H, Zhou H, Ge Y, Xiao L, Hu X, Liu BH, Yang J, Li YY, Chen S, Eaves CJ, Wu D, Zhao Y. Oncogene. 2014 Aug 4.
Caffeic Acid phenethyl ester inhibits epithelial-mesenchymal transition of human - Caffeic Acid phenethyl ester inhibits epithelial-mesenchymal transition of human
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c-Myc expression and MEK1-induced Erk2 nuclear localization are required for - c-Myc expression and MEK1-induced Erk2 nuclear localization are required for
Amatangelo MD, Goodyear S, Varma D, Stearns ME. Carcinogenesis. 2012 Oct;33(10):1965-75.
Dietary polyphenol quercetin targets pancreatic cancer stem cells. - Dietary polyphenol quercetin targets pancreatic cancer stem cells.
Zhou W, Kallifatidis G, Baumann B, Rausch V, Mattern J, Gladkich J, Giese N, Moldenhauer G, Wirth T, Buchler MW, Salnikov AV, Herr I. Int J Oncol. 2010 Sep;37(3):551-61.
Epithelial-mesenchymal transition induced by growth suppressor p12CDK2-AP1 - Epithelial-mesenchymal transition induced by growth suppressor p12CDK2-AP1
Tsuji T, Ibaragi S, Shima K, Hu MG, Katsurano M, Sasaki A, Hu GF. Cancer Res. 2008 Dec 15;68(24):10377-86.