Anti-TXNIP antibody

• Supplier: Abcam
• Catalog: ab86983
• Prices: $394.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Rat, Human, Mouse, Pig, Orangutan
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human TXNIP
• Description: Rabbit Polyclonal
• Gene: TXNIP
• Conjugate: Unconjugated

Product images

All lanes : Anti-TXNIP antibody (ab86983) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab86983 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86983, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab86983 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86983, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

NLRP3 gene silencing ameliorates diabetic cardiomyopathy in a type 2 diabetes rat - NLRP3 gene silencing ameliorates diabetic cardiomyopathy in a type 2 diabetes rat

Luo B, Li B, Wang W, Liu X, Xia Y, Zhang C, Zhang M, Zhang Y, An F. PLoS One. 2014 Aug 19;9(8):e104771.

TXNIP links innate host defense mechanisms to oxidative stress and inflammation - TXNIP links innate host defense mechanisms to oxidative stress and inflammation

Devi TS, Lee I, Huttemann M, Kumar A, Nantwi KD, Singh LP. Exp Diabetes Res. 2012;2012:438238.