Anti-U1A antibody
| Name | Anti-U1A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55751 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-283 of Human U1A |
| Description | Mouse Monoclonal |
| Gene | SNRPA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
U1A antibody (ab55751) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart tissue.
ab55751 at 10 ug/ml staining U1A in human Hella cells by Immunocytochemistry/ Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab55751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55751, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16888_U1A-Primary-antibodies-ab55751-2.jpg)
Overlay histogram showing HeLa cells stained with ab55751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55751, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in - The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in
Ammon T, Mishra SK, Kowalska K, Popowicz GM, Holak TA, Jentsch S. J Mol Cell Biol. 2014 Aug;6(4):312-23.
The transition in spliceosome assembly from complex E to complex A purges surplus - The transition in spliceosome assembly from complex E to complex A purges surplus
Hodson MJ, Hudson AJ, Cherny D, Eperon IC. Nucleic Acids Res. 2012 Aug;40(14):6850-62.
Splicing-independent recruitment of U1 snRNP to a transcription unit in living - Splicing-independent recruitment of U1 snRNP to a transcription unit in living
Spiluttini B, Gu B, Belagal P, Smirnova AS, Nguyen VT, Hebert C, Schmidt U, Bertrand E, Darzacq X, Bensaude O. J Cell Sci. 2010 Jun 15;123(Pt 12):2085-93.