All lanes : Anti-U1A antibody [EPR10632] (ab166890) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : SH SY5Y cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Western blot analysis (using ab166890 at 1/10000 dilution) of pellet from Jurkat cell lysate immunoprecipitated using ab166890 at 1/10000 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of 293T cells labeling U1A with ab166890 at a 1/50 dilution.