Immunofluorescent analysis of paraformaldehyde-fixed U87-MG cells labeling U2AF1L3 with ab188582 at 1/250 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
All lanes : Anti-U2AF1L3 antibody [EPR14349] (ab188582) at 1000 Lane 1 : Mouse brain lysateLane 2 : PC-12 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-U2AF1L3 antibody [EPR14349] (ab188582) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human cerebellum lysateLane 3 : K562 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution