Anti-U2AF35 antibody [EPR12648(2)]

• Supplier: Abcam
• Catalog: ab197591
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12648(2)
• Applications: ICC/IF ICC/IF FC IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human U2AF35 aa 50-150
• Description: Rabbit Monoclonal
• Gene: U2AF1
• Conjugate: Unconjugated

Product images

Anti-U2AF35 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-U2AF35 antibody [EPR12648(2)] (ab197591) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-U2AF35 antibody [EPR12648(2)] (ab197591) at 1/1000 dilution + Rat spleen lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma) cells labeling U2AF35 with ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.