U2AF1

Gene Symbol U2AF1
Entrez Gene 7307
Alt Symbol FP793, RN, RNU2AF1, U2AF35, U2AFBP
Species Human
Gene Type protein-coding
Description U2 small nuclear RNA auxiliary factor 1
Other Description U2 small nuclear RNA auxillary factor 1|U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit|U2 snRNP auxiliary factor small subunit|U2(RNU2) small nuclear RNA auxiliary factor binding protein|splicing factor U2AF 35 kDa subunit|splicing factor U2AF 35kDa subunit
Swissprots Q01081 Q71RF1 Q701P4
Accessions BAA95534 EAX09501 EAX09502 EAX09503 EAX09504 EAX09505 EAX09506 Q01081 AB451244 BAG70058 AF370386 AAQ15222 AJ627978 CAF29556 AK022152 AK023589 AK094435 AK098266 AK307560 AL832665 CAH10401 BC001177 AAH01177 BC001923 AAH01923 BC005915 AAH05915 BC013786 BC032521 CB110007 HQ447764 ADQ32249 M96982 AAA36619 XM_011529743 XP_011528045 NM_001025203 NP_001020374 NM_001025204 NP_001020375 NM_006758 NP_006749
Function Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. {ECO:0000269|PubMed:8647433}.
Subcellular Location Nucleus {ECO:0000269|PubMed:15096518}. Nucleus speckle {ECO:0000269|PubMed:15096518}.
Top Pathways Spliceosome

U2AF1 (D6S3Q) Rabbit mAb - 13705 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6S3Q
Western blot analysis of extracts from various cell lines using U2AF1 (D6S3Q) Rabbit mAb.

U2AF35 (C-12) - sc-19663 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
U2AF35 (C-12): sc-19663. Western blot analysis of U2AF35 expression in HEK293 whole cell lysate.

U2AF35 (N-16) - sc-19961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
U2AF35 (N-16): sc-19961. Western blot analysis of U2AF35 expression in HeLa (A), HEK293 (B), Ramos (C) and Hep G2 (D) whole cell lysates and HeLa (E) and NIH/3T3 (F) nuclear extracts.

U2AF1L3/35 (D-4) - sc-514459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
U2AF1L3/35 (D-4): sc-514459. Western blot analysis of U2AF1L3/35 expression in WI-38 (A), HeLa (B) and NAMALWA (C) whole cell lysates and IMR-32 nuclear extract (D).

U2AF1L3/35 (H-151) - sc-368532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-U2AF35 antibody - ab177071 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-U2AF35 antibody (ab177071) at 1/500 dilution + 293T cell lysate

Anti-U2AF35 antibody - ab80164 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-U2AF35 antibody (ab80164) at 1/50 dilution + Ramos cell line lysates at 35 µg

Anti-U2AF35 antibody [EPR12648(2)] - ab197591 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12648(2)
Anti-U2AF35 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-U2AF35 antibody [EPR12649(B)] - ab172614 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12649(B)
All lanes : Anti-U2AF35 antibody [EPR12649(B)] (ab172614) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : NIH 3T3 cell lysateLane 4 : Ramos cell lysateLysates/proteins at 10 µg per lane.

Anti-U2AF1 Antibody (aa190-240) - LS-C288841 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-U2AF1 Antibody - LS-C341451 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-U2AF1 Antibody - LS-C331218 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using U2AF1 antibody.

Anti-U2AF1 Antibody (clone 1C8) - LS-C198390 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C8
Detection limit for recombinant GST tagged U2AF1 is 0.1 ng/ml as a capture antibody.

Anti-U2AF1 Antibody (N-Terminus) - LS-C110505 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
U2AF1 antibody LS-C110505 Western blot of Fetal stomach lysate.

Anti-U2AF1 Antibody - LS-C334507 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using U2AF1 antibody.

Anti-U2AF1 Antibody (aa140-190) - LS-C288840 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse U2AF35 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti-U2AF35 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-U2AF1 Antibody (aa140-190) - LS-C286762 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse U2AF35 by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma. Antibody: Affinity purified rabbit anti-U2AF35 used at a dilution of 1:250.

Anti-U2AF1 Antibody (aa99-125) - LS-C97291 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-U2AF1 Antibody (aa55-83) - LS-C97290 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with U2AF1 antibody (Center S70), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

U2AF35 Antibody - NBP1-19120 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.

U2AF35 Antibody - NBP1-32515 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.

U2AF35 Antibody - NBP1-92553 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-92553] Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.

U2AF35 Antibody (1C8) - H00007307-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C8
Sandwich ELISA: U2AF35 Antibody (1C8) [H00007307-M01] - Detection limit for recombinant GST tagged U2AF1 is 0.1 ng/ml as a capture antibody.

Anti-U2AF1 (center S70) antibody produced in rabbit - SAB1300700 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-U2AF1 (Center S70) Antibody: <B>Cat. No. SAB1300700</B>: U2AF1 in human hepatocarcinoma tissue with Anti-U2AF1 (Center S70) Antibody at 40 μg/mL.

Anti-U2AF1, (N-terminal) antibody produced in rabbit - SAB2104536 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-U2AF1, antibody produced in rabbit: <B>Cat. No. SAB2104536</B>: Immunoblotting analysis of U2AF1 in fetal stomach tissue lysate with U2AF1 anitbody at 1 μg/mL.
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