All lanes : Anti-U2AF35 antibody [EPR12649(B)] (ab172614) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : NIH 3T3 cell lysateLane 4 : Ramos cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling U2AF35 with ab172614 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling U2AF35 with ab172614 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells labeling U2AF35 with ab172614 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab172614 at 1/10 dilution.