Anti-U2AF65 antibody

• Supplier: Abcam
• Catalog: ab37483
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Human, Mouse, Bovine, Xenopus, Zebrafish
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human U2AF65
• Description: Rabbit Polyclonal
• Gene: U2AF2
• Conjugate: Unconjugated

Product images

All lanes : Anti-U2AF65 antibody (ab37483) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab37483 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37483, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

IHC image of U2AF65 staining in human lymphoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37483, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product References

The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in - The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in

Ammon T, Mishra SK, Kowalska K, Popowicz GM, Holak TA, Jentsch S. J Mol Cell Biol. 2014 Aug;6(4):312-23.

The in vivo kinetics of RNA polymerase II elongation during co-transcriptional - The in vivo kinetics of RNA polymerase II elongation during co-transcriptional

Brody Y, Neufeld N, Bieberstein N, Causse SZ, Bohnlein EM, Neugebauer KM, Darzacq X, Shav-Tal Y. PLoS Biol. 2011 Jan 11;9(1):e1000573.