U2AF2

Gene Symbol	U2AF2
Entrez Gene	11338
Alt Symbol	U2AF65
Species	Human
Gene Type	protein-coding
Description	U2 small nuclear RNA auxiliary factor 2
Other Description	U2 (RNU2) small nuclear RNA auxiliary factor 2\|U2 auxiliary factor 65 kDa subunit\|U2 small nuclear ribonucleoprotein auxiliary factor (65kD)\|U2 snRNP auxiliary factor large subunit\|hU2AF65\|splicing factor U2AF 65 kDa subunit
Swissprots	Q96HC5 P26368
Accessions	EAW72402 EAW72403 EAW72404 P26368 AB451261 BAG70075 AB451387 BAG70201 AK311672 BC008740 AAH08740 BC030574 AAH30574 CN364862 DQ592840 JF432180 ADZ15397 X64044 CAA45409 XM_006722994 XP_006723057 XM_011526410 XP_011524712 NM_001012478 NP_001012496 NM_007279 NP_009210
Function	Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. {ECO:0000269\|PubMed:15009664, ECO:0000269\|PubMed:19470458, ECO:0000269\|PubMed:19574390, ECO:0000269\|PubMed:21536736}.
Subcellular Location	Nucleus.
Top Pathways	Spliceosome

U2AF65 (D-8) - sc-374333 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

U2AF65 (D-8): sc-374333. Western blot analysis of U2AF65 expression in HeLa (A) and SK-N-SH (B) whole cell lysates.

U2AF65 (H-300) - sc-48804 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

U2AF65 (H-300): sc-48804. Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.

U2AF65 (MC3) - sc-53942 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

U2AF65 (MC3): sc-53942. Western blot analysis of U2AF65 expression in HeLa whole cell lysate (A) and Jurkat (B), SK-N-MC (C) and KNRK (D) nuclear extracts.

U2AF65 (C-8) - sc-166695 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

U2AF65 (C-8): sc-166695. Western blot analysis of U2AF65 expression in HeLa (A) and Jurkat (B) nuclear extracts.

U2AF65 (N-14) - sc-19958 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

U2AF65 (N-14): sc-19958. Western blot analysis of U2AF65 expression in Jurkat (A), IMR-32 (B), KNRK (C), HeLa (D) and SK-N-MC (E) nuclear extracts.

Anti-U2AF65 antibody - ab37530 from Abcam

Prices	$400.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-U2AF65 antibody (ab37530) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.

Anti-U2AF65 antibody - ab37483 from Abcam

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-U2AF65 antibody (ab37483) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.

Anti-U2AF65 antibody - ab151582 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF65 antibody (ab151582) at 1/10000 dilution + A431 cell lysate at 30 µg/ml

Anti-U2AF65 antibody - ab177052 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinoma tissue labeling U2AF65 with ab177052 at 1/100 dilution.

Anti-U2AF65 antibody - ab131014 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-U2AF65 antibody (ab131014) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-U2AF65 antibody [EPR17046] - C-terminal - ab197031 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17046

All lanes : Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/20000 dilutionLane 1 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-U2AF65 antibody [MC3] - ab50684 from Abcam

Prices	$372.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	MC3

Anti-U2AF2 / U2AF65 Antibody - LS-C18407 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-U2AF2 / U2AF65 Antibody (N-Terminus) - LS-C31659 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30, Azide-free, HRP) - LS-C272823 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30, APC) - LS-C272820 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30, FITC) - LS-C272822 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30, AP) - LS-C272819 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa371-420) - LS-C116802 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate.

Anti-U2AF2 / U2AF65 Antibody (aa1-30, Biotin) - LS-C272821 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30, PE) - LS-C272824 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa380-430) - LS-C290324 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa50-100) - LS-C290320 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa430-475) - LS-C290325 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-U2AF2 / U2AF65 Antibody (aa1-30) - LS-C160361 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

U2AF2 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The U2AF2 antibody detected the U2AF2 protein (arrow).

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