All lanes : Anti-UAP56 antibody [EPR13144] (ab181059) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling UAP56 using ab181059 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling UAP56 using ab181059 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181059 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.