All lanes : Anti-UAP56 antibody [EPR13144-62] (ab181061) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human infiltrating duct breast carcinoma tissue sections labeling UAP56 using ab181061 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181061 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
Flow Cytometry analysis of Jurkat cells labeling UAP56 using ab181061 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.