Anti-UBA52 antibody [EPR4547]

• Supplier: Abcam
• Catalog: ab109230
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4547
• Applications: WB IP IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human UBA52
• Description: Rabbit Monoclonal
• Gene: UBA52
• Conjugate: Unconjugated

Product images

ab109230 stained HepG2 cells. The cells were 4%  formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilutionLane 1 : Human placenta lysateLane 2 : Raji cell lysateLane 3 : Fetal kidney lysateLane 4 : 293T cell lysateLane 5 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue, using ab109230 at a 1/500 dilution.