All lanes : Anti-Ube1L antibody - Azide free (ab167663) at 1/500 dilutionLane 1 : Ube1L transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG at 1/2500 dilutiondeveloped using the ECL technique
Anti-Ube1L antibody - Azide free (ab167663) at 1/500 dilution + Human liver lysate at 50 µgSecondaryGoat Anti-Mouse IgG developed using the ECL technique
Immunofluorescence analysis of HeLa cells, labeling Ube1L with purified ab167663 at 10 µg/ml.