Anti-UBE2C antibody
| Name | Anti-UBE2C antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12290 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide derived from within residues 150 to the C-terminus of Human UBE2C |
| Description | Rabbit Polyclonal |
| Gene | UBE2C |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Image courtesy of Human Protein Atlas ab12290 stainning UBEC2 in the nucleus of surface epithelial cells of human esophagus. Paraffin-embbeded tissue was incubated with ab12290 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab12290 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-UBE2C antibody (ab12290) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : HEK293 Human embryonic kidney cell line Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
ICC/IF image of ab12290 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab12290, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 and MCF7 cells.
Product References
Gene expression profiling of mouse p53-deficient epidermal carcinoma defines - Gene expression profiling of mouse p53-deficient epidermal carcinoma defines
Garcia-Escudero R, Martinez-Cruz AB, Santos M, Lorz C, Segrelles C, Garaulet G, Saiz-Ladera C, Costa C, Buitrago-Perez A, Duenas M, Paramio JM. Mol Cancer. 2010 Jul 14;9:193.
Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on - Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on
Lin J, Raoof DA, Wang Z, Lin MY, Thomas DG, Greenson JK, Giordano TJ, Orringer MB, Chang AC, Beer DG, Lin L. Neoplasia. 2006 Dec;8(12):1062-71.