ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):Lane 1 = IP from Noc arrested HeLa cell extract (beads only).Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)Lane 4 = Noc arrested HeLa whole cell lysate.For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
ab3935 at a 1/200 dilution staining human UBE2C in paraformaldehyde fixed HeLa cells by Immunocytochemistry. In the upper panel G2 phase cells can be seen (G1 cells stained only faintly). The lower panel shows a mitotic (metaphase) cell. Diffuse cytoplamic staining with some microtubule localisation can be observed in mitotic cells.
