All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : Rat spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/1000 dilution (unpuified)Lane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD