UBE3A

Gene Symbol UBE3A
Entrez Gene 7337
Alt Symbol ANCR, AS, E6-AP, EPVE6AP, HPVE6A
Species Human
Gene Type protein-coding
Description ubiquitin protein ligase E3A
Other Description CTCL tumor antigen se37-2|E6AP ubiquitin-protein ligase|human papilloma virus E6-associated protein|human papillomavirus E6-associated protein|oncogenic protein-associated protein E6-AP|renal carcinoma antigen NY-REN-54|ubiquitin-protein ligase E3A
Swissprots Q9UEP7 Q9UEP9 Q9UEP8 Q93066 P78355 Q05086 A8K8Z9 Q9UEP4 Q9UEP5 Q9UEP6
Accessions AAB69154 CAA04534 CAA04535 CAA04536 CAA04537 CAA04538 CAA04539 CAA04540 CAA66653 EAW57642 EAW57643 EAW57644 EAW57645 Q05086 AB194232 BAD69553 AF002224 AF002225 AF273050 AAG34910 AI378869 AK056239 AK130491 AK291405 BAF84094 AK292514 BAF85203 AL049363 AL831842 BC002582 AAH02582 BC009271 AAH09271 BE349076 DA415876 JF432815 ADZ16032 L07557 AAA35542 U84404 AAB49301 X98031 CAA66654 X98032 CAA66655 X98033 CAA66656 XM_005268267 XP_005268324 XM_005268268 XP_005268325 XM_005268269 XP_005268326 XM_005268270 XP_005268327 XM_005268271 XP_005268328 XM_006720673 XP_006720736 XM_006720674 XP_006720737 XM_006720675 XP_006720738 XM_006720676 XP_006720739 XM_011521994 XP_011520296 XM_011521995 XP_011520297 XM_011521996 XP_011520298 NM_000462 NP_000453 NM_130838 NP_570853 NM_130839 NP_570854
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). {ECO:0000269|PubMed:10373495, ECO:0000269|PubMed:19204938, ECO:0000269|PubMed:19233847, ECO:0
Subcellular Location Nucleus {ECO:0000250|UniProtKB:O08759}. Cytoplasm {ECO:0000250|UniProtKB:O08759}.
Top Pathways Ubiquitin mediated proteolysis, Viral carcinogenesis

UBE3A (D10D3) Rabbit mAb - 7526 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10D3
Western blot analysis of extracts from various cell lines using UBE3A (D10D3) Rabbit mAb.

E6-AP (C-19) - sc-18114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
E6-AP (C-19): sc-18114. Western blot analysis of E6-AP expression in K-562 (A) and Ramos (B) whole cell lysates.

E6-AP (E-4) - sc-166689 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
E6-AP (E-4): sc-166689. Western blot analysis of E6-AP expression in HeLa (A), K-562 (B), Jurkat (C), Ramos (D), SK-N-MC (E), NIH/3T3 (F) and KNRK (G) whole cell lysates.

E6-AP (C-18) - sc-8927 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

E6-AP (E-5) - sc-166532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
E6-AP (E-5): sc-166532. Western blot analysis of E6-AP expression in IMR-32 nuclear extract (A) and SK-N-SH whole cell lysate (B).

E6-AP (F-1) - sc-166688 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
E6-AP (F-1): sc-166688. Western blot analysis of E6-AP expression in HeLa (A), K-562 (B), Jurkat (C), Ramos (D) and SK-N-MC (E) whole cell lysates.

E6-AP (G-18) - sc-12380 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
E6-AP (G-18): sc-12380. Western blot analysis of E6-AP expression in Jurkat whole cell lysate.

E6-AP (K-16) - sc-23778 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

E6-AP (H-182) - sc-25509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
E6-AP (H-182): sc-25509. Western blot analysis of E6-AP expression in IMR-32 (A), HeLa (B) and SK-N-MC (C) nuclear extracts and HeLa whole cell lysate (D).

E6-AP (N-14) - sc-8926 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
E6-AP (N-14): sc-8926. Western blot analysis of E6-AP expression in mouse brain tissue extract.

Anti-UBE3A antibody - ab10488 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBE3A antibody (ab10488) at 1/1000 dilutionLane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-UBE3A antibody - ab3519 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE3A antibody - ab94493 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBE3A antibody (ab94493) at 1 µg/mlLane 1 : T24/83 (Human bladder carcinoma) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-UBE3A antibody - ab124389 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-UBE3A antibody (ab124389) at 1/500 dilution + Human fetal liver lysate

Anti-UBE3A antibody - ab183869 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded mouse middle brain tissue, labeling UBE3A using ab183869 at a 1/500 dilution.

Anti-UBE3A antibody - ab58266 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
UBE3A antibody (ab58266) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.

Anti-UBE3A antibody - ab10487 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab10487 immunoprecipitation of  UBE3A (E6AP). Samples: Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody: ab10487 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is UBE3A (E6AP).

Anti-UBE3A antibody [EPR7330] - ab126765 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7330
All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : Rat spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-UBE3A antibody [EX-8] - ab195649 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone EX-8

Anti-E6AP / UBE3A Antibody (aa1-851) - LS-C19980 from LifeSpan Bioscience

Prices $295.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-E6AP / UBE3A Antibody - LS-C48667 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-E6AP / UBE3A Antibody - LS-C65144 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-E6AP / UBE3A Antibody - LS-C65145 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-E6AP / UBE3A Antibody (C-Terminus) - LS-C123428 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-E6AP / UBE3A Antibody (aa843-873, APC) - LS-C225265 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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