Anti-UBF1 antibody [EP2741Y]

• Supplier: Abcam
• Catalog: ab75781
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP2741Y
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to residues near the N-terminus of human UBF1
• Description: Rabbit Monoclonal
• Gene: UBTF
• Conjugate: Unconjugated

Product images

Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-UBF1 antibody [EP2741Y] (ab75781) at 1/500 dilutionLane 1 : (A) HeLa cell lysateLane 2 : (B) A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.