UBTF

Gene Symbol UBTF
Entrez Gene 7343
Alt Symbol NOR-90, UBF, UBF-1, UBF1, UBF2
Species Human
Gene Type protein-coding
Description upstream binding transcription factor, RNA polymerase I
Other Description 90-kDa nucleolus organizer region autoantigen|autoantigen NOR-90|nucleolar transcription factor 1
Swissprots A8K6R8 P17480
Accessions AAC51239 CAC34632 EAW51616 EAW51617 EAW51618 EAW51619 EAW51620 P17480 AF289595 AAL55779 AK291733 BAF84422 AK292518 BAF85207 AK292577 BAF85266 AK296917 BAG59472 AK298008 BAG60313 AK308321 BC031423 AAH31423 BC042297 AAH42297 BP230502 DR000113 M61724 X53390 CAA37469 X53461 CAA37548 X56687 CAA40016 XM_006722059 XP_006722122 XM_006722060 XP_006722123 XM_006722061 XP_006722124 XM_011525177 XP_011523479 NM_001076683 NP_001070151 NM_001076684 NP_001070152 NM_014233 NP_055048 NR_045058
Function Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. {ECO:0000269|PubMed:7982918}.
Subcellular Location Nucleus, nucleolus {ECO:0000250}.

UBF (F-9) - sc-13125 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBF (F-9): sc-13125. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

UBF (H-300) - sc-9131 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
UBF (H-300): sc-9131. Western blot analysis of UBF expression in A-431 nuclear extract.

UBF (K-17) - sc-12427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBF (K-17): sc-12427. Western blot analysis of UBF expression in PMA-induced KNRK nuclear extract.

Anti-UBF1 (phospho S484) antibody - ab138677 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-UBF1 (phospho S484) antibody (ab138677) at 1/500 dilution + extracts from HeLa cells treated with calyculin A 50ng/ml for 30 minutes.

Anti-UBF1 (phospho S484) antibody [EPR2725(2)] - ab182583 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2725(2)
All lanes : Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583) at 1/10000 dilutionLane 1 : Nocodazole treated HeLa cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-UBF1 antibody - ab166841 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBF1 antibody (ab166841) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : Jurkat cell extractLane 3 : Jurkat cell extract with synthesized peptide

Anti-UBF1 antibody - ab61205 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-UBF1 antibody (ab61205) at 1 µg/ml + HepG2 whole cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-UBF1 antibody - ab65059 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBF1 antibody (ab65059) at 1/500 dilutionLane 1 : Extracts from HeLa cells at 5-30ugLane 2 : Extracts from Jurkat cells at 5-30ugLane 3 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug

Anti-UBF1 antibody - ab112964 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-UBF1 antibody (ab112964) at 1/100 dilution + K562 cell line lysates at 35 µg

Anti-UBF1 antibody [EP2741Y] - ab75781 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2741Y
Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-UBF1 antibody [EPR2742] - ab109011 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2742
All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : MCF7 cell lysateLane 4 : JAR cell lysateLysates/proteins at 10 µg per lane.

Anti-UBTF / UBF Antibody (aa631-660, APC) - LS-C272974 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa631-660, AP) - LS-C272975 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa631-660, Biotin) - LS-C272976 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (N-Terminus) - LS-C169539 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa631-660, FITC) - LS-C272977 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa631-660) - LS-C160402 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
UBTF Antibody western blot of K562 cell line lysates (35 ug/lane). The UBTF antibody detected the UBTF protein (arrow).

Anti-UBTF / UBF Antibody (aa631-660, Azide-free, HRP) - LS-C272978 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa631-660, PE) - LS-C272980 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa546-596) - LS-C288639 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (phospho-Ser484) - LS-C291653 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (aa551-651, Azide-free) - LS-C329026 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-UBTF / UBF Antibody (aa630-660) - LS-C326161 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBTF / UBF Antibody (N-Terminus, clone EP2741Y) - LS-C105366 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2741Y
IHC of paraffin-embedded human breast carcinoma using anti-UBF1 antibody (LS-C105366).

Anti-UBTF / UBF Antibody (Glu536) - LS-C176737 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of UBF-1 (E536) pAb in paraffin-embedded human brain tissue.
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