Anti-UBN1 antibody [UBN1-02]

• Supplier: Abcam
• Catalog: ab84953
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: UBN1-02
• Applications: FC WB IP
• Species Reactivities: Human
• Antigen: Recombinant fragment, corresponding to N terminal amino acids 1-190 of Human UBN1
• Description: Mouse Monoclonal
• Gene: UBN1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab84953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84953, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Lane 1 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 1 µg/mlLane 2 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 5 µg/mlLane 1 : HeLa nuclear lysateLane 2 : HeLa nuclear lysate