![Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)Lanes 3 - 4 : Ponceau S RedLane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNALane 2 : U2OS were co-transfected with mismatch controlLane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNALane 4 : U2OS were co-transfected with mismatch controlObserved band size : 54 kDa (why is the actual band size different from the predicted?)Additional bands at : 49 kDa (possible isoform).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/17672_UBXN6-Primary-antibodies-ab80659-2.jpg)
Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)Lanes 3 - 4 : Ponceau S RedLane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNALane 2 : U2OS were co-transfected with mismatch controlLane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNALane 4 : U2OS were co-transfected with mismatch controlObserved band size : 54 kDa (why is the actual band size different from the predicted?)Additional bands at : 49 kDa (possible isoform).